Regulatory
ErmE

Part:BBa_K1968008

Designed by: Ibrahim Al-Masoud   Group: iGEM16_Edinburgh_OG   (2016-10-12)

ErmE promoter Phytobrick


A widely used constitutive promoter, ErmE (promoter region of erythromycin-resistance gene) was isolated from Streptomycin (Bibb et al., 1985). It works with related gram positive actinobacteria, such as Rhodococcus. We have this part in pUPD2, a MoClo-compatible level 0 vector with no illegal sites for MoClo assembly standard, GoldenBraid assembly standard and iGEM’s pairwise assembly standard.

References

Bibb, M.J., Janssen, G.R. and Ward, J.M., 1985. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene, 38, pp.215-226.

Tong, Y., Charusanti, P., Zhang, L., Weber, T. and Lee, S.Y., 2015. CRISPR-Cas9 based engineering of actinomycetal genomes. ACS synthetic biology, 4, pp.1020-1029.


ErmE promoter Phytobrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None